The MCF facility provides access and support to modern flow cytometry analysis of cells or particles and their physical separation via sorting. Our conventional, spectral and imaging devices allow high-dimensional multicolor flow cytometry and cell sorting based on total fluorescence signals as well as cells morphology and fluorescence intracellular patterns (link to Equipment). Any suspended particle or cell between 0.2 and 150 µm is suitable for analysis and between 0.2 and 30 µm for cell sorting in our facility. Typical applications include immunophenotyping, cell cycle and proliferation assays, measurement of cell viability and apoptosis, analysis of membrane and intracellular proteins/antigens, mitochondrial membrane potential assays as well as cell sorting for various downstream applications such as in vitro cultures, clonal lines, biochemical assays, or sequencing. 

The MCF Flow Cytometry service is open to external users from academia and industry (pricelist link).  

For more information, please contact Katarzyna Misztal (kmisztal@iimcb.gov.pl). 

For sample preparation, see our guidelines (link to sample prep). 

We offer:

Full service

• Consultation, design of experiments
• Sample acquisition (analysis)
• Cell sorting
• Preliminary data analysis and visualization (in FlowJo)
• Preparation of figures (in FlowJo)

Note: we do not offer cell culturing and staining’s

Equipment access

• Cell sorters training
• BD FACSAria II (3-4 session) using beads or User samples
• CytoFLEX SRT (1-2 session) using beads or User samples
• Analyzers are only available with an operator