The MCF electron microscopy service offers analyses of cells, tissues, and virus particles with a FEI Tecnai T12 transmission electron microscope. For the conventional transmission electron microscopy of cells and tissue samples, we use a Leica EM tissue processor. This enables resin processing under constant temperature while avoiding exposure to toxic substances. After saturation with resin, tissue and cell specimens are pretrimmed with a Leica EM TRIM2, which prepares for the next step of processing. Samples are then cut into semi- and ultra-thin sections using a Leica EM UC7 ultramicrotome, and then sections are placed on electron microscopy grids. Material that is prepared this way can be imaged with our electron microscope. Typical applications include ultrastructure visualization, pre and post embedding immunostaining as well as co-localization based on PLA (Proximity Ligation Assay).

The MCF Electron Microscopy service is chargeable to both internal and external users according to the pricelist.

For more information, please contact Matylda Macias (mmacias@iimcb.gov.pl) and Aleksandra Szybinska (aszybinska@iimcb.gov.pl).

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We offer:

Full service

• Consultation, design of experiment, quotation
• Samples preparation
  • Ultrastructure (fixation in 2.5% glutar aldehyde)
  • Ultrastructure and immunogold staining: pre-embedding EM (fixation in 4% PFA, immunostaining, fixation in 2.5 glutar aldehyde)
• Sample acquisition
• Data analysis and visualization
• Preparation of figures