The MCF facility provides access and support for flow cytometry analysis of cells or particles and their physical separation via sorting. Our 5-lasers analyzers and two high-end cells sorters allow multiparametric/multicolor flow cytometry, cell sorting from cell cultures and tissues as well as single cell sorting to the multi-well plates and microscopic slides(check here). Any suspended particle or cell between 0.2 and 150 µm is suitable for analysis and between 0.2 and 30 µm for cell sorting in our facility. Typical applications include immunophenotyping, cell cycle and proliferation assays, measurement of cell viability and apoptosis, analysis of membrane and intracellular proteins/antigens, mitochondrial membrane potential assays as well as cell sorting for various downstream applications such as in vitro cultures, clonal lines, biochemical assays, or sequencing.

The MCF Flow Cytometry service is free of charge for internal researchers. External users from academia and industry are charged according to the pricelist.

For more information, please contact Katarzyna Misztal (kmisztal@iimcb.gov.pl).

For sample preparation see our guidelines.

We offer:

Full service

• Consultation, design of experiments
• Sample acquisition (analysis)
• Cell sorting
• Preliminary data analysis and visualization (in FlowJo)
• Preparation of figures (in FlowJo)

Note: we do not offer cell culturing and staining’s

Equipment access

• Cell sorters training
• BD FACSAria II (3-4 session) using beads or User samples
• CytoFLEX SRT (1-2 session) using beads or User samples
• Analyzers are only available with an operator