The MCF is equipped with Tecnai T12 Transmission Electron Microscope (TEM) for sample imaging as well as other devices necessary for sample preparation for electron microscopy (see below). Users can access TEM only with assistance from the facility. All details of sample preparation and fixation for TEM must be agreed with the facility each time.

Tecnai T12 Transmission Electron Microscope

Contact: Matylda Macias

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General Description

Tecnai T12 is combined with the TemCam F-Series camera and is supported by MCF for conventional imaging of cells, tissue samples or viral particles. Users can access the microscope only with facility assistance.

Leica EM TP Tissue Processor

Contact: Matylda Macias

https://mcf.iimcb.gov.pl/wp-content/uploads/2023/08/EM-TP-640x640.jpg

General Description

The Leica EM TP enables automated sample preparation for electron (EM) and light microscopy (LM). It is the first tissue processor to feature a heating/cooling system with pre-heat and pre-cool capabilities, in a closed processing chamber, to maintain a constant temperature while avoiding exposure to toxic substances. The processor carousel holds 24 EM or 12 LM vials and utilizes various specialized baskets and capsules.

Leica EM TRIM2 Trimmer

Contact: Matylda Macias

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General Description

The Leica EM TRIM2 is a high-speed milling system with an integrated stereomicroscope and LED ring illuminator for trimming biological and industrial samples prior to ultramicrotomy. The Leica TRIM2 features 1µm feed control for the milling cutter, orthogonal view of deposited sample to determine the position for accurate milling.

Leica EM UC7 Ultramicrotome

Contact: Matylda Macias

https://mcf.iimcb.gov.pl/wp-content/uploads/2023/08/EM-UC7-640x640.jpg

General Description

The Leica EM UC7 Ultramicrotome enables the easy preparation of semi- (~300 nm thick) and ultrathin (~ 60 nm thick) sections and perfect, smooth surfaces of samples for TEM examination. It operates with broad feed range from 1 nm up to 15 µm and fully motorized knife stage plus “autotrim” function to keep the highest quality of specimen preparation.